The characterisation of family-13 kinesins in Trypanosoma brucei

Followers
0

Organisation

University of Hull

There is no description for this organisation

License

© Kuan Yoow Chan. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder.

The characterisation of family-13 kinesins in Trypanosoma brucei

Kinesins are motor proteins involved in the movement of organelles and sub-organelles along microtubule tracks within the cell. Phylogenetic analysis of the 46 kinesin genes coded by the Trypanosoma brucei genome resulted in the grouping of seven kinesin sequences into the Kinesin-13 family. Members of this family have been characterised in a number of model organisms and, unlike most kinesins, do not exhibit microtubule processivity and are capable of depolymerising microtubules. They play important roles in bipolar spindle assembly and chromosome segregation. Of the six T. brucei Kinesin-13 proteins that were characterised during this study, only one was found to have a nuclear localisation, while the rest were found localised to the mitochondrion, cell body or flagellum. Attempts to probe the function of these kinesins using RNAi resulted in a reduction of cell growth in three of the six kinesins studied, but no gross changes in cellular morphology were observed. The distinct localisation of five Kinesin-13 family members outside the nucleus suggests that the functional diversity of the Kinesin-13 family is larger than previously thought.

Data and Resources

Core Fields

Field Value
Metadata Created 27 January 2016, 18:11 (UTC)
creator
creator_identifier
creator_identifier_scheme
creator_affiliation
title_type
subject
subject_scheme
subject_scheme_identifier
keywords
geo_location
geo_location_point
geo_location_box
geo_location_place
format
unique_resource_identifier
publisher
publisher_identifier
project
funder_name
funder_id
date
date_type
rights
license_name
license_uri
access
resource_type
resource_type_additional
contact
contact_email
language
related_identifier
related_identifier_type
relation_type
image_thumbnail
metadata_created
metadata_license

Additional Info

Field Value
Maintainer Department of Biological Sciences, The University of Hull
Last Updated 22 May 2017, 15:47 (UTC)
http_access_status All Resources Available

Citation

The characterisation of family-13 kinesins in Trypanosoma brucei. oai-hull-ac-uk-hull-1361